Interested in making your own agar plates for mycology work? Below are some simple agar recipes you can make at home.

Agar plates can help you to clean up spore prints, make mushroom clones, isolate mushroom genetics, and much more. Not only is it super fun, but using agar plates for mushroom work lets you learn the basics of mycelial formation and the mushroom lifecycle.

If you are ready to level up your mushroom game, agar work is the ticket.

agar recipe for mushroom work

Below are 7 agar recipes used for taking mushroom cultures, genetic isolations, cloning, and spore germination.

The recipes listed below will make approximately 20 90mm Petri dishes. Adjust accordingly if you need more or less. 

Agar Recipies for Fungal Growth

Malt Extract Agar Recipe (MEA)

Potato Dextrose Agar Recipe (PDA)

Buy PDA Premix Here

Malt Extract Agar Recipe with Yeast and Peptone (MAYP)

Potato Dextrose Agar Recipe with Yeast and Peptone (PDAYP)

Malt Extract Agar Recipe with Yeast, Peptone, and Activated Carbon  (Black Agar)

Potato Dextrose Agar Recipe with Yeast, Peptone, and Activated Carbon  

Antibiotic Agar Recipe (AB)

Antibiotic Agar is an agar medium (usually MEA or PDA) that has been infused with an antibiotic for the purposes of combating bacterial contamination. There are many different antibiotics you may use, most of which require a prescription. However, some of these ingredients can be purchased over the counter for the purpose of treating pets, i.e. fish antibiotics  

Important note: Antibiotics are heat sensitive so it is important to add the antibiotic of your choice after sterilization as the agar is cooling. Allow the agar to cool to around 55°C before adding in the antibiotic.


  • 500 ml Distilled H20
  • 10g Agar powder
  • 10g Malt Extract Powder
  • Antibiotic of choice (choose one from the list below, add after sterilization, and swirl around)
    • Levaquin 500 mg
    • Doxycycline 500 mg
    • Amoxicillin 500 mg
    • Ciprofloxacin 500 mg
    • Cephalexin 500 mg
    • Azithromycin 500 mg

Do you have an Agar recipe that works great for you? If so, share with the community by posting your agar recipe in the comments below!