How to Make Liquid Culture: A Step-by-Step Guide


Mushroom cultivation has been a burgeoning field, attracting hobbyists, farmers, and researchers alike. While the prospect of growing your own mushrooms is enticing, the process can be intricate, particularly when it comes to understanding and nurturing the mycelium—the vital root system of fungi. This article demystifies the process of creating a liquid mycelium culture, an essential step in mushroom cultivation, providing a step-by-step guide and addressing common concerns.

What is Liquid Mycelium Culture?

A liquid mycelium culture is a nutrient-rich solution in which the mycelium, the vegetative part of a fungus, can grow and propagate. It is created by introducing a small amount of mycelium into a sterile liquid nutrient medium, where it can grow and be stored for future use or directly inoculated into a substrate suitable for mushroom growth.

liquid culture jar

Importance of Liquid Mycelium Culture

Liquid mycelium culture is integral to mushroom cultivation for several reasons. Firstly, it allows for the rapid colonization of substrate, thereby speeding up the overall cultivation process. Secondly, it provides a cost-effective way to propagate a large quantity of mycelium from a small sample. Lastly, it can be stored for extended periods, making it a reliable reserve for continued mushroom cultivation.

The Role of Mycelium in Mushroom Cultivation

Mycelium: The Root Network of Fungi

Mycelium serves as the lifeblood of fungi, akin to the root system of a plant. It’s a vast, intricate network of thread-like cells called hyphae, which traverse the growth substrate—be it soil, wood, or a specific cultivation medium—absorbing nutrients, and eventually giving rise to the fruiting bodies we know as mushrooms.

Advantages of Cultivating Mycelium

Cultivating mycelium provides a head start in the mushroom growth process. By nurturing a healthy, robust mycelium network, you ensure that the resulting mushrooms will be of high quality. Furthermore, mycelium cultures can be stored and used to inoculate new batches of substrate, making them a sustainable solution for continued cultivation.

Materials Needed for Making Liquid Mycelium Culture

Liquid Culture Recipe

The recipe for making a liquid culture broth is simple. It requires a sugar concentration of 4%. Light malt extract is commonly preferred as it is easy to measure, but other sugars or starches like Karo, dextrose, or corn syrup can also be used. Here’s a standard recipe:

Alternatively, you may purchase premade agar powder such as this LC Premix from Evviva Sciences.

Required Tools

Step-by-Step Guide to Making Liquid Mycelium Culture

Preparing the Ingredients

Start by measuring your dry ingredients using a precision scale. Measure the distilled water and pour it into a mixing container. Next, add the light malt extract (and brewers yeast if you’re using it) to the water and mix, creating your liquid culture broth.

Tip: Bringing the water to a boil before mixing in the dry ingredients will help dissolvability. Do this either by microwaving the water or on the stovetop. Some users choose to strain large particles using a coffee filter before sterilizing for a cleaner-looking liquid culture.

Sterilizing the Media

Half-fill each mason jar with the liquid culture broth, add a glass marble or a magnetic stir rod to assist in breaking up clumps of mycelium later on, and cover with a modified lid and a piece of aluminum foil. Sterilize the jars in a pressure canner for 20 minutes at 15PSI. Let it cool completely before proceeding.

Inoculating the Media

Next, introduce the mycelium into your sterilized media. This can be done using a healthy agar culture or liquid culture that contains the desired mycelium. You can do this with a sterile instrument, transferring a small amount of the agar culture or a few drops of the liquid culture into the jar.

Agitation and Oxygenation

Once your mycelium is inoculated, it’s crucial to agitate the liquid culture jar daily to increase oxygenation. Be careful not to splash liquid near the lid to prevent potential contamination. A magnetic stirrer can be used for this purpose. This ensures the mycelium is evenly distributed throughout the solution, which promotes the growth and health of the mycelium.

Monitoring the Growth

The time it takes for a liquid culture to colonize depends on several factors, including the species of mushroom, temperature, and the type of nutrients used. On average, it can take anywhere from a few days to several weeks for a liquid culture to fully colonize. Regular monitoring of the growth is essential.


Creating a liquid mycelium culture might seem daunting at first, but with the right knowledge, tools, and a little patience, it is an achievable task. Cultivating your own mycelium not only provides a cost-effective and sustainable way to grow mushrooms but also offers a unique opportunity to appreciate the intricate and fascinating lifecycle of fungi.

Frequently Asked Questions

1. What is the ideal temperature for growing a liquid mycelium culture?

Different species of mushrooms require different temperatures for optimal growth. However, in general, most mushroom species grow best at temperatures between 20-24°C (68-75°F).

2. How long can I store a liquid mycelium culture?

Stored properly in a refrigerator, a liquid mycelium culture can last for several months.

3. Can I use any type of sugar for the liquid culture broth?

While light malt extract is most commonly used due to its ease of measurement, other sugars or starches such as Karo or corn syrup can also be used.

4. Why is agitation important in a liquid mycelium culture?

Agitation ensures that the mycelium is evenly distributed throughout the solution, promoting the growth and health of the mycelium.

5. Can I use a liquid mycelium culture to grow any type of mushroom?

Yes, you can grow different types of mushrooms using a liquid mycelium culture. However, the specific growth parameters and time it takes for the culture to colonize will vary depending on the mushroom species.

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